today we are discussing about condition that influence the anti heroes of microbes so let’s do it. Destruction of micro organism and inhibition of microbial growth are note simple as a number of factors affect the efficiency of an antimicrobial agent. It is not possible to find simple agent that can be effectively used for control of micro organisms for all material and in all circumstances it is necessary to evaluate each situation to select a process which will give the desired results.
Some of the condition that influence the efficiency of antimicrobial agents are
- Environment
The environment in which microorganisms are present i.e. the physical and chemical properties of mediums or substances in which microorganisms are suspended has a great influence on rate and efficiency of microbial destruction. Heat is more effective in killing microorganisms in acidic rather than alkaline material. The penetration power of agents depends on consistency of material . high concentration of carbohydrates increases the thermal resistance of microorganisms. Efficiency of antimicrobial agents is decreased in the presence of extraneous organic matter
The presence of organic matter like blood, serum, Plant or animal tissues. Mucus etc can significantly reduce the efficiency of the antimicrobial agent because of the following reasons.
- It may combine with the organic matter to form a product which is not microbicidal eg. the agents get inactivated.
- It may combine with the organic matter to form a precipitate. Thus the interaction between the agent and organisms is prevented.
- Accumulation of the organic matter on the microbial cell surface may provide a coating which will also prevent the interaction between the agent and microbial cell
2) Killing of microorganisms
Different microorganisms differ in their susceptibility to physical and chemical agents. Spores are more resistant than growing vegetative cells in spore forming species. Bacterial spores are the most resistant of all living organisms in their ability to survive adverse physical and chemical conditions.
3) Physiological state of cells
The age of microbial culture markedly influences the susceptibility of the antimicrobial agent. Young actively metabolizing cells are destroyed more easily as compared to old dormant cells by an agent that causes damage by interfering in the metabolism. And non growing cells are not affected.