microbiology notes about e.coli

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E.coli morphology :

• Gram negative bacilli
• Straight rod shaped
• Arranged single or in pair
• Motile by peritrichous flagella
• Non sporting
• Aerobes and facultative anaerobes

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Antigen :

• “O” Antigen
  • Lipopolysaccharide ag
  • Heat stable
  • Early “O” serotype – normal commensal – intestine
  • Late “O” serotype – diarrhoea production
  • Protect bacteria from phagocytes
• “H” antigen
  • Flagellar ag
  • Motile
  • Heat labile
• “K” antigen
  • Polysaccharide capsular Ag
  • Inhibits phagocytosis
• Fimbria Ag:
  • Attachment and colonization

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Toxins :

• Enterotoxin :
  • Cytotoxic necrotizing factor 1
  • Produces diarrhoea
• Heat labile toxin
• Heat stable toxin
• Verocytoxin

Clinical features :

• UTI

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